Sweena Chaudhari

I finished my studies in Virology but decided to switch to Immunology, a field which inspired and excited me tremendously. My PhD focused on the role of dendritic cells in atherosclerosis at the University of Wuerzburg, Germany. I later moved across the Atlantic ocean to work as a Postdoctoral fellow at The Scripps research institute, Florida and understand T-cell subtypes and their nitty gritty pathway switches. I am currently working on a biotech startup in India and understanding the tremendous challenges it brings about.

Articles by Sweena Chaudhari:

How to Fool-“Proof” Your Experiment: An Introduction to Yeast Plasmids

A lot of research experiments require the use of a eukaryotic host as opposed to E. coli due to its greater conformity and suitability in expressing eukaryotic proteins. This is the reason why yeast cells have gained importance as cloning and expression hosts. For protein expression studies to hybrid screens, many applications require insertion and…

23 Jan 2019 Cells and Model Organisms

DNA Footprinting

Studying DNA–protein interactions is an important aspect of molecular biology. Researches use a variety of methods to study these like the chromatin immunoprecipitation (ChIP) assay, electrophoretic mobility shift assay (EMSA), DNA pull down assay, luciferase reporter assay, filter binding assay, yeast one hybrid system, etc. Another interesting assay that helps investigate DNA–protein interactions is the…

09 Mar 2018 DNA / RNA Manipulation and Analysis

Guide to Making and Storing Competent Yeast Cells

Yeasts, such as Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris, are routinely used in biology research labs around the world. Yeasts are easy-to-culture, unicellular eukaryotes, and make excellent model organisms because of the similarity of their genes and proteins with those of their mammalian counterparts. Yeast cells are used to study gene function, protein interactions,…

02 Nov 2017 Cells and Model Organisms

The Inhibitors Haunting Your PCR

It often happens that you do everything right with a PCR. You have perfectly isolated template DNA, used sterile tubes and tips, used clean reagents, and said a quick prayer to the PCR Gods. And still, something unknown messes up your results. This unknown at work is generally a PCR inhibitor. Before you blame it…

27 Jun 2017 PCR, qPCR and qRT-PCR&Qiagen

Cre-loxP Recombination Essentials Part 2

The Cre-loxP recombination system is routinely used for the generation of mouse knockouts. In part 1 of this mini-series, I introduced the concept and applications of Cre-loxP. As with any other technology or research tool, it has limitations and pitfalls that need to be considered while planning experiments or interpreting results. This article will take…

21 Jun 2017 Cells and Model Organisms&Lab Statistics & Math