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Sweena Chaudhari

I finished my studies in Virology but decided to switch to Immunology, a field which inspired and excited me tremendously. After a PhD working on the role of dendritic cells in immune regulation of atherosclerosis at the University of Wuerzburg, Germany, I have moved across the Atlantic ocean for newer experiences. I am currently a Postdoctoral fellow at The Scripps research institute, Florida and work with T-cell subtypes and their nitty gritty pathway switches.

Articles by Sweena Chaudhari:

Guide to Making and Storing Competent Yeast Cells

Yeasts, such as Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris, are routinely used in biology research labs around the world. Yeasts are easy-to-culture, unicellular eukaryotes, and make excellent model organisms because of the similarity of their genes and proteins with those of their mammalian counterparts. Yeast cells are used to study gene function, protein interactions,…

02 Nov 2017 Cells and Model Organisms

The Inhibitors Haunting Your PCR

It often happens that you do everything right with a PCR. You have perfectly isolated template DNA, used sterile tubes and tips, used clean reagents, and said a quick prayer to the PCR Gods. And still, something unknown messes up your results. This unknown at work is generally a PCR inhibitor. Before you blame it…

27 Jun 2017 PCR, qPCR and qRT-PCR

Cre-loxP Recombination Essentials Part 2

The Cre-loxP recombination system is routinely used for the generation of mouse knockouts. In part 1 of this mini-series, I introduced the concept and applications of Cre-loxP. As with any other technology or research tool, it has limitations and pitfalls that need to be considered while planning experiments or interpreting results. This article will take…

21 Jun 2017 Cells and Model Organisms&Lab Statistics & Math

Cre-loxP Recombination Essentials Part 1

You might have heard of the Cre-loxP system even if you are not directly working with genetic manipulation. The Cre-loxP system is an ubiquitous technology for genetic manipulation and a mainstay in mouse research labs. With this system you can delete genes in cells, specific tissues and even whole organisms! You can start to master this system by…

01 Jun 2017 Cells and Model Organisms

Laboratory Animal Ethics: The Three Rs

Animal models have helped make enormous discoveries and breakthroughs in the last few decades. From Pasteur’s use of sheep to test the ‘Germ Theory’, Pavlov’s classic conditioning experiments in dogs, to Dolly the first cloned mammal, animal research has come a long way. Today, most drugs, vaccines and other pharmaceutical products for medical use are…

21 Feb 2017 Cells and Model Organisms