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Mike Millar

Mike has a certificate in Management, Business Administration & Management from The University of Northampton and is currently Histology & Imaging Research Facility Manager, University of Edinburgh and Chief Technical Officer, Aquila BioMedical.

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Articles by Mike Millar

Image of a mechanic thinking before fixing a car to represent thinking before performing histology fixation

An Introduction To Fixation For Histology: Think Before You Fix!

By Mike Millar | August 9, 2021

How you fix your tissue or cells can affect your results, for better or for worse. Discover the key points to think about before undertaking your histology fixation.

Can’t See the Mouse for the Mice?! Solutions to Mouse-on-Mouse Immunohistochemistry Detection

Can’t See the Mouse for the Mice?! Solutions to Mouse-on-Mouse Immunohistochemistry Detection

By Mike Millar | March 18, 2014

The issue of mouse-on-mouse background is only a cause for concern for the histotechnologist working within a research environment. Those working in a diagnostic setting will probably never experience this as they will be working with human tissue with antibodies raised in a variety of species- but one species that won’t be used is human!…

Image of glow sticks to represent Fluorescent Tyramides In Histology

Fluorescent Tyramides In Histology: A Versatile Approach For Multiplex Molecular Tissue Probing

By Mike Millar | August 21, 2013

Fluorescent tyramides offer exquisite sensitivity, are easy to use, and are versatile. Find out how to use them to brighten up your research.

Immunohistochemistry- PAP, APPAP and Sandwiches!

By Mike Millar | February 12, 2013

You’ll give me an (enzymatic) complex! Following on from Part 1 of this article, let’s start by having a look at the two most popular enzymatic ‘sandwich’ methods; The Peroxidase anti Peroxidase method (PAP). The PAP method was the first sandwich method that I used and involves three main stages- application of primary antibody, secondary…

Immunohistochemistry- Direct vs. Indirect Methods, and a Golden Rule

Immunohistochemistry- Direct vs. Indirect Methods, and a Golden Rule

By Mike Millar | January 29, 2013

In my previous article I covered different immunohistochemical staining techniques at a superficial level. In the following articles I will start to explain these technologies in a bit more detail and in which situations they should be applied. All of the following will involve additional stages when applying them, for example- serum blocking, protein blocking,…

Immunohistochemistry- an Introduction, Techniques and an Evolution Towards Robots!

Immunohistochemistry- an Introduction, Techniques and an Evolution Towards Robots!

By Mike Millar | January 15, 2013

The last two decades have seen a dramatic increase in the number of publications using immunohistochemistry (IHC) as a research tool to identify the spatial location of proteins of interest within cells, tissue sections and whole-mount preparations. Grinding and binding The advantages over ‘grind and bind’ methods are apparent, but the very best results will…

Tissue Embedding and Sectioning: Something to Think About Whilst in the Bath.

Tissue Embedding and Sectioning: Something to Think About Whilst in the Bath.

By Mike Millar | October 16, 2012

In the same way that you should ‘Think Before You Fix’, the choice of embedding media should be dictated by your required end-point. The basic principle is that by processing tissue into an embedding medium you harden the tissue and provide support protecting it from the mechanical forces associated with sectioning. Parma ham and steak…

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