In my last article, I talked about the basic protocols and experiments conducted in the process of converting algae into biofuel. Our ability to culture algae has efficiently improved over the years. Continuous improvisation in basic techniques has helped us to understand the growth limiting step of algae culture.
In this article, I will discuss some fundamental techniques to culture algae.
Different Types of Systems Available for Microalgae Culture
Open System
One of the most natural methods to culture algae is in a free environment (sunny areas are preferred) to get maximum production. Considering their low maintenance and construction costs, open ponds are the best options for conducting initial experiments on a large scale.
There are different parameters you need to consider carefully before building an open pond. Bad weather and constantly changing water temperatures can inhibit high production. For this reason, open pond systems are usually built where temperatures range higher than 15°C. Also, the entry of foreign organisms can compete with algae for common growth limiting substrates. Furthermore, evaporative losses, uneven light intensity, and diffusion of CO2 in the atmosphere are some major limitations of open ponds.
Open systems are categorized into natural (small ponds, lakes, lagoons) and artificial ponds (container, tanks). The most popular open pond system is the artificial raceway pond in which nutrients, algae, and water are regulated along a circular path. You can build a raceway pond simply by digging a recess into the ground, lining it with plastic to prevent the absorption of the nutrient solution into the earth, and adding a paddlewheel to circulate the water. Ponds vary in size from 0.5m2 to 100m2; a single paddlewheel provides sufficient mixing for a 5ha cultivation area.
What to Consider When Designing an Open System
There are many different factors to consider when designing an open system. The most important are:
Mixing patterns: mixing plays an important role in the distribution of sunlight and other essential nutrients, but limited research has been conducted to study mixing characteristics and flow patterns. To begin, conduct experiments to optimize mixing characteristics based on continuous or semi-continuous mixing, speed, time duration, etc. Different experiments should be conducted to optimize these mixing characteristics.
Mixing technology: mixing can be achieved using different technologies. For example, gas sparging and magnetic beads have been used in algae ponds. The type of technology you choose will depend on your requirements and scale.
Depth of open ponds should be considered along with mixing characteristics such as circulation velocity to have uniform culture density. Try the above points in different depths of open ponds that range from 5-100 cm.
Computational Fluid Dynamics/kinetic Studies: You should perform these studies to determine the physical, hydrological, geometrical, and dynamic variables that predict the behavior of the open pond system
You should also keep in mind several other important parameters such as the extent of water evaporation rate, contaminations, and external influences.
All the abovementioned points vary according to experiment requirements, strain, environmental conditions, etc.
Closed System
A closed system, also called a photobioreactor (PBR), provides a controlled environment to culture algae. The closed system environment can be manipulated according to species requirements. Every parameter, including carbon dioxide level, water supply, temperature, light intensity, culture density, pH level, aeration rate, and mixing pattern can be controlled.
A basic, closed system bioreactor.
Since PBRs function in controlled circumstances, (e.g. uniform temperature and light intensity) higher and continuous productivity is achieved. However, the capital cost and technical aspects in sterilizing the system prevent the use of photobioreactors on a large scale.
Setting Up Your Own Photobioreactor
Since all experimental conditions are controlled by you, and photobioreactors can be purchased, setting up a closed system is easier than setting up an open system. To start your experiments, you should:
Construct and test the photobioreactor under different mixing conditions.
Different conditions can be altered to optimize your system, including:
Using different size bottles (height and width) in different racks.
Covering the water bottles with different colored sheets (red, green, blue, yellow) that absorb different wavelengths of light.
Varying the diffusion rate of carbon dioxide using a pump.
Currently, algae farms are unsustainable on a large scale due to many limiting factors such as high media cost, energy intensive and continous production. However, with increases in advancements in technology and culture techniques, extensive assessment is going on to increase the efficiency of the production sector.
The good news is that there is still a lot of room for improvisation in algae farming.
Sonication is mostly used during preparation of protein extracts to help break apart the cell. Although most lysis buffers have buckets of detergent that lyse cell membranes, sonication just gives an extra hand in breaking everything apart. Sonication also breaks up, or shears, DNA in a sample—preventing it from interfering with further sample preparation. Have…
Struggling with your PhD advisor relationship? Sometimes its repairable and sometimes it’s better to switch. Discover how to identify when it’s time to switch advisors and steps you can take to ensure a smooth successful change to keep your research and academic goals stay on track.
There are several yeast transformation protocols around, and most of them require a lot of steps: overnight starter culture, dilution and growth to logarithmic phase, several washes, and so on… These protocols work very well since they have been optimised for maximum transformation efficiency, which is needed for applications like library construction. But they are…
If you do cell culture you will inevitably need to count your cells. Counting cells can be tedious, but it is important to do accurately. Your assessed quantity of living cells will affect all your downstream applications. In this article I will not only cover how to manually count your cells and how to do…
Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other international scientists. The problem is that I don’t know how to address the situation. If they indeed have antibodies that…
As a researcher, it’s satisfying to manage your own projects and do the bench work yourself. After all, if you don’t have experience with a technique, you’re usually expected to figure it out (with or without direct supervision). In some situations, dealing with difficult molecular techniques is simply part of the job description. The scientific…
10 Things Every Molecular Biologist Should Know
The eBook with top tips from our Researcher community.