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Basically, you choose a gel based on two main factors: how high you need the resolution to be and what is in your samples. To make the choice simpler, I made a handy chart describing the differences between them.
*Pulsed field electrophoresis is a technique in which the direction of current flow in the electrophoresis chamber is periodically altered. This allows fractionation of pieces of DNA ranging from 50, 000-5 millon bp.
Use this chart to help you understand the differences between the two gel types and your samples will be running like athletes in no time!
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Top 10 Tips for Proper Pipette Care
A neglected tool can easily play havoc with your experiments and ruin results. Download our handy poster to get top tips on proper pipette care. Stick it up in the lab to help remind yourself and any fellow lab mates how to look after this vital lab tool.
This ebook introduces you to some of the most common safety principles that you will encounter every time you step into a laboratory. On top of that, it will help point you towards additional resources to answer your questions when deeper questions arise.
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Once upon a time, I thought reproducible research meant if someone else showed X in a paper, then I should be able to get X in my experiment. However, this actually refers to replication, an important but separate concept. Reproducible research is data analysis that starts with the raw data and arrives at the same…
Gel and blot imaging has come a long way. Film gave you sensitivity but slowed you down with darkrooms and inconsistent results. Camera systems sped things up but still left you fighting noise and limited dynamic range. Laser imaging now gives you cleaner signals, sharper focus, and far more flexibility across sample types.
Here’s an easy guide on how to maintain your lab balance. Following these tips will ensure your balance remains clean and perhaps most importantly, accurate.
One of our readers posted the following question to us and we decided to pass it along to everybody’s favorite microbiology expert, Aunt Yersinia: For one year I am working in different research laboratories, after I got from school. I keep wondering why EVERYBODY is using pre-made Agar solutions for pouring plates, and EVERYBODY is…
Using gloves in the lab is necessary for safety but can result in the dreaded “sweaty glove hands”. Read our top tips on how to banish those sweaty palms for good (or for at least as long as it takes to do your experiment)!
Ever wonder why your data isn’t the same after repeating an experiment? Well part of science’s beauty lies in the difficulty of achieving reproducibility. Heraclitus first said that no mans steps in the same river twice and the same can be applied to experiments. It is literally impossible to control for everything because the second…
10 Things Every Molecular Biologist Should Know
The eBook with top tips from our Researcher community.
