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Basically, you choose a gel based on two main factors: how high you need the resolution to be and what is in your samples. To make the choice simpler, I made a handy chart describing the differences between them.
*Pulsed field electrophoresis is a technique in which the direction of current flow in the electrophoresis chamber is periodically altered. This allows fractionation of pieces of DNA ranging from 50, 000-5 millon bp.
Use this chart to help you understand the differences between the two gel types and your samples will be running like athletes in no time!
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EBOOK
Guide to Lab Safety
This ebook introduces you to some of the most common safety principles that you will encounter every time you step into a laboratory. On top of that, it will help point you towards additional resources to answer your questions when deeper questions arise.
Keeping your pipette clean and sparkling is critical. After all a contaminated pipette could ruin your precious samples in the lab! Download our cheat sheet to get a simple guide to cleaning your pipette. Stick it up in the lab to help remind yourself how to look after this vital lab tool.
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Messing up the initial calculations in your experiment is setting yourself up for failure. Here is a quick refresh on calculating the number of molecules in any DNA sample.
So you grab a quick 5 minutes in between lectures to sit down and tackle an item on your to-do list: order a secondary antibody for an upcoming experiment. But when you start to search your favorite secondary antibody provider’s website, you realize it is not going to be a 5 minute job. Conjugated, F(ab’)2…
When I was in school, I absolutely hated giving talks. I was a really nervous presenter, my heart would start beating faster, my face would go red, my hands would shake…even my voice would tremble! Since then I’ve made some big breakthroughs, and now I absolutely love giving talks and lectures. Here are my top…
It seems like every movie that needs a shot of a scientist doing their sciencey-thing either gets the person to pour one pretty liquid from one flask into another or to stare intensely at a test tube with a look of knowing so much more than you ever could. Another favorite shot is of someone…
On the surface, it would seem easy enough to pick an enzyme (or an amount of enzyme) for an experiment. Just look at the concentration on the label, adjust accordingly, and you’re on your way. Alas, not with enzymes. The number of units used to measure enzymes is dizzying. However, it’s better now than it…
10 Things Every Molecular Biologist Should Know
The eBook with top tips from our Researcher community.