Basically, you choose a gel based on two main factors: how high you need the resolution to be and what is in your samples. To make the choice simpler, I made a handy chart describing the differences between them.
*Pulsed field electrophoresis is a technique in which the direction of current flow in the electrophoresis chamber is periodically altered. This allows fractionation of pieces of DNA ranging from 50, 000-5 millon bp.
Use this chart to help you understand the differences between the two gel types and your samples will be running like athletes in no time!
As a researcher, it’s satisfying to manage your own projects and do the bench work yourself. After all, if you don’t have experience with a technique, you’re usually expected to figure it out (with or without direct supervision). In some situations, dealing with difficult molecular techniques is simply part of the job description. The scientific…
Did you know that there are many alternatives to the animal-derived products we use in the lab? Read more for how and why you might want to make the switch.
When you work in a laboratory, preparing samples sets for many different experiments is a large part of the job. Keeping track of your samples can be tedious or even challenging if you don’t already have a good system in place. However, getting this right is a critical part of the experimental process. In this…
As biological catalysts, enzymes transform target substrates into products. Enzyme kinetics is the rate of that transformation. By understanding how an enzyme’s behavior is affected, you can figure out how it functions in physiology or fails to function in disease. Now it gets complicated… What Affects an Enzyme’s Kinetics? In the first place, most enzymes…
Using gloves in the lab is necessary for safety but can result in the dreaded “sweaty glove hands”. Read our top tips on how to banish those sweaty palms for good (or for at least as long as it takes to do your experiment)!
A lot of chemical reagents are relatively non-hazardous.But there are just as many that are extremely hazardous, which means you’ll want to take precautions to reduce any risk of exposure, repeated exposure, and of course, accidental contamination of anything – or anyone – that walks out of the lab at the end of the day….
10 Things Every Molecular Biologist Should Know
The eBook with top tips from our Researcher community.
