Equipment for photographing DNA gels stained with ethidium bromide (or other fluorescent dyes), doesn’t have to cost thousands of dollars. These days, great pictures can be obtained with a standard digital camera and an orange filter. Here’s how.
You will need:
A digital camera
A Cokin orange filter 002A or similar (This does not have to fit onto the camera, a square filter will do.)
A polystyrene ice bucket with a thick (3-5cm bottom)
A UV transilluminator
To build it:
Cut a hole in the bottom of the box that is big enough for the camera lens to fit into.
Tape the filter over the hole, inside the box.
Set the camera flash to off and the mode to black & white.
Push the camera’s lens tube into the hole (or place the camera lens over the hole if there is no lens tube). Depending on the shape of your camera, you may have to modify the box to ensure that the camera is pointing straight into the hole.
Place your DNA gel onto the transilluminator and put the box over the gel.
Turn the transilluminator and the camera on. You should now be able to see the stained DNA on the gel in the viewfinder of the camera.
To get a quality picture and good detection limits you will have to play around with the ISO, shutter speed and aperture. This will be specific to your camera, but the setting ISO 200, Shutter speed 1/3, Aperture 8.0 worked for me so may be a useful starting point for you.
Update: Here’s a schematic diagram that shows how the whole thing should fit together. Let me know if you have any comments or questions.
My PhD is rapidly becoming a distant memory. Before nostalgia completely obscures my recollections of this chapter of my career, I thought I’d jot down some pointers for prospective and current PhD students. These are mainly based on things I wish I had done during my PhD, or mistakes I have seen others make. I […]
It’s great to have you in the Bitesize Bio family! We’ve sent you an email to confirm your registration. Please click on the link in the email or paste it into your browser to finalize your registration.
For more information on how to use Bitesize Bio, take a look at the following image (click it, for a larger version)
An error occured while registering you, please reload the page and try again