A Beginner’s Guide to Next Generation Sequencing (NGS) Technology

A Beginner’s Guide to Next Generation Sequencing (NGS) Technology

The completion of the Human Genome Project in 2003 ushered in a new era of rapid, affordable, and accurate genome analysis—called Next Generation Sequencing (NGS). NGS builds upon “first generation sequencing” technologies to yield accurate and cost-effective sequencing results. Fred Sanger sequenced the first whole DNA genome, the virus phage ?X174, in 1977. In that…

Digital PCR or Quantitative Real-Time PCR: Which Method Is Best for Your Quantitative PCR Application?

Digital PCR or Quantitative Real-Time PCR: Which Method Is Best for Your Quantitative PCR Application?

So you’re designing a new experiment that requires PCR quantification. You used to have only one method to choose from, but now you have two – Quantitative Real-Time PCR (qPCR) and Digital PCR (dPCR). Which one is right for your application? Both methods have good quantification, sensitivity and specificity for most applications. They are compatible…