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Jelena Jankovic

Jelena is a Research Associate with PhD in biochemistry, working in the field of thyroid oncology with a focus on molecular diagnostics. Freelance scientific writer contributing articles on lab hacks and survival in the life science field.

During her PhD, she investigated the diagnostic and prognostic potential of protein and genetic biomarkers for thyroid cancer. She continues to focus my research on improving early diagnosis of thyroid cancer while moving my research towards exploring molecular markers in liquid biopsies. I have mastered several molecular biology techniques and acquired skills in statistical data analysis and data interpretation. Specific fields of interest include mutation analysis, gene expression analysis, and statistical modeling. As a freelance writer, she contributes to a popular website for life scientists and enjoys the opportunity to learn more about presenting complicated matters in a simple way. She plans to expand her knowledge in both communicating science and providing high-value content.

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Articles by Jelena Jankovic

Protein Colocalization: You Know They Overlap, but Can You Prove It?

Protein Colocalization: You Know They Overlap, but Can You Prove It?

By Jelena Jankovic | May 3, 2018

Microscopy by default is a technique that allows us to observe, rather than measure, biological events and make conclusions based on what we see, rather than based on some calculations. The topic of this article may therefore seem a little surprising since it deals with the measurements of colocalization. While there are situations where you…

microRNA

Extracting Circulating microRNA, Part Two: Isolation Methods

By Jelena Jankovic | March 21, 2018

In the second part of this two part series on analyzing extracellular microRNA (miRNA) from blood or serum, we continue with the methods for miRNA extraction. Generally, there are two different approaches for microRNA isolation. The first of these is organic, liquid-liquid. The total RNA extraction method employs phenol/chloroform isolation with guanidine isotiocyanate as a…

microRNA

Extracting Circulating microRNA, Part One: The Preanalytical Phase

By Jelena Jankovic | March 5, 2018

microRNA (miRNA) is leading the way as circulating biomarkers in disease biomarker discovery. The hype around circulating miRNA is warranted. They meet many criteria for a good biomarker—namely, stability in circulation and varying levels in certain pathological conditions. Recently, studies have shown that miRNAs could be useful markers for cancer, myocardial infarction, atherosclerosis, and neuro-degenerative…

droplet digital PCR

Divide and Conquer: How to Setup Your First Droplet Digital PCR Experiment

By Jelena Jankovic | April 6, 2017

Droplet digital PCR?  It’s easy. Because we’re here to guide you through it. We recently introduced you to the principles of digital PCR technology and how it differs from qPCR. In a nutshell, digital PCR is an end-point PCR technology that divides a single PCR into a large number of partitions, and then perform PCR…

Reverse Transcription: The Most Common Pitfalls!

Reverse Transcription: The Most Common Pitfalls!

By Jelena Jankovic | January 19, 2017

Good quality starting material is king for reverse transcription! Obtaining reliable results in any experiment requires good preparation. We often take reverse transcription for granted, and we don’t always consider that our qPCR might be performing poorly because of problems in that step. Since it’s quite often the reverse transcription reaction itself that causes fuss…

acid phenol chloroform

Acid Phenol Chloroform Extraction of DNA, RNA and protein: 3 in 1

By Jelena Jankovic | November 23, 2016

In austerity times, nothing is in excess. Apart from saving reagents, which can be refilled with extra financial injections, there is a commodity that cannot be easily resupplied – tissue samples! If, like me, you have experienced the fear of not having enough sample for performing a qPCR, western blot, and conventional PCR from the…

relative quantification

Methods for Relative Quantification of qPCR Data. Yes, There is More Than One.

By Jelena Jankovic | August 18, 2016

As all of you probably know, methods for calculating relative gene expression from qPCR data include: a) double delta Ct (ΔΔCt) and b) that one other method. Chances are you’ve probably gotten beyond the ΔΔCt method, but you should be prepared in case you face primer sets of different amplification efficiencies. Both methods require the…

Choose the Statistical Package that Will Make Your Data Talk

Choose the Statistical Package that Will Make Your Data Talk

By Jelena Jankovic | July 9, 2016

In the last years, the need for using statistical testing in bioscience has grown exponentially and so has the development of statistical software. It is now common that everyone is using some sort of stats in their basic research. Among the skillful biostatisticians, R is the most popular software for data analysis, but not all…

R coding

Online Schools for Learning How to Play in the R Coding Arena

By Jelena Jankovic | July 9, 2016

So, you come from a non-coding background, but given the rapid growth of your research results, you have accrued a pile of data that needs data mining. Now, you can leave this to your bioinformatics core facility, but if you’re curious to understand how they arrive at their conclusions (or you don’t have a BI…

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