Search results for: CRISPR
An Introduction to Chimeric Antigen Receptors (CARs)
…and Faster CAR: Seatbelts, Airbags, and CRISPR. Biol Blood Marrow Transplant. Jan:24(1):27–31. Riviere I, Sadelain M (2017). Chimeric Antigen Receptors: A Cell and Gene Therapy Perspective. Molecular Therapy. May 3;25(5):1117–24….
Read MoreC. elegans: The Elegant Model System
…mostly have single copies of genes in their genome, so no question of redundancy. More recently, easy and targeted genome editing methods like mosSCI, TALEN and CRISPR/Cas9 have been developed…
Read MoreThose Site-Specific Recombinases in Your Tool Kit
Most of us are aware of genetic engineering systems like Cre-Lox, TALENs, Zinc finger systems, and of course, CRISPR-Cas9. These are all examples of CSSR- Conservative Site-Specific Recombination. We use…
Read MoreHow to Prevent False Results in Colony PCR
…also cloning projects for which the insert is not PCR amplified. For example, inserts can be made by annealing synthetic oligonucleotides (as is the case in many CRISPR/Cas9 guide RNA…
Read MoreWhere To Get Your Perfect Cell Line
…know-how, especially which methods are most likely to be successful for your target. For example, CRISPR isn’t efficient across the entire genome, so depending on where your target region is,…
Read MoreDefend Science Funding! A Brief Guide
…to come. Consider incorporating discussions of science funding into formal outreach events and into everyday conversations with friends and family. Consider CRISPR, which began as an investigation into bacterial defense…
Read MoreNGS Target Enrichment Strategies
…in gene editing with the emergence of CRISPR/Cas9 enzymology. With this approach, Cas9 endonuclease can be guided by an RNA molecule to a homologous DNA sequence on a genome and…
Read MoreA Short History of Cell Biology
…insight into transcriptomics at the resolution of individual cells. 2009 – First paper published using organoids derived from a single adult stem cell. 2012 – CRISPR gene editing is developed,…
Read MoreAAV Production Part I: Tips for Setting Up
…is rapidly diluted within the culture and may also be actively degraded. However, a non-permanent transduction may be useful for some applications, such as CRISPR. AAV is also an excellent…
Read MoreDNA Extraction for Next Gen Sequencing
…novel twist. The agarose gel electrophoresis is semi-automated, and CRISPR/Cas9 technology (a process called CATCH) is used to target extract specific HMW genomic regions up to 500 kb. This can…
Read MoreSite-Directed Mutagenesis Tips and Tricks
…approaches are gaining traction over site-directed mutagenesis. Furthermore, the emergence of CRISPR/Cas9 technology has also simplified gene editing such that mutagenesis can now be performed in vitro and in vivo…
Read More5 Ways to Improve Your Fluorescent Protein Sorting
If, like many people, you use fluorescent proteins to view your transfection efficiency or your CRISPR gene editing, you can also isolate cells with different expression levels of fluorescent proteins….
Read MoreSix Facts About Restriction Enzymes
…technique in which restriction enzymes are used, CRISPR-Cas9 genome editing is a newer technique employing another type of restriction. Cas9 protein is an integral part of the complex prokaryotic immune…
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