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last updated: December 3, 2019
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Macrophages are a type of white blood cell derived from monocytes that are most widely known for the ability to phagocytose cell debris, pathogens, and even cancer cells. However, it is becoming clear that the role of macrophages goes beyond eliminating cellular waste. Macrophages are often used in conjunction with T cells to measure immune…
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You walk into your tissue culture room to find a window open, the incubator’s humidity tray empty and a pipette lying on its side in the hood on a used glove… After you have found and torn to shreds the person responsible for this monstrous act (!!), consider posting the below tips to help those…
Biosafety cabinets keep your work safe from contamination, and you safe from your work. So here are 12 tips for Working in a biosafety cabinet!
There’s more to mammalian cell culture than just making sure that your cells don’t die. It is a lot like taking care of children. You have to feed them, make sure that they’re growing well, and keep them under constant supervision. If the cells are put through extreme conditions (over-confluency, media-deprivation, inaccurate temps, etc.), their…
As the vast majority of bacteria cannot be readily cultured in the laboratory [1], culture-dependent methods to investigate bacteria grossly underestimate the diversity of bacterial communities. To investigate unculturable bacteria without isolating them, culture-independent methods such as sequencing have been used. Unculturable bacteria can be identified by PCR amplification and sequencing of housekeeping genes such…
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