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You had been happily measuring your DNA samples with your NanoDrop for a while, until… you noticed that your results are not reproducible *gulp*. Read on – here are 10 points that will help you troubleshoot the issue.
last updated: September 7, 2021
Farania Rangkuti is pursuing a PhD in Evolutionary Ecology of Infectious Disease at the University of Oxford.
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Microarrays are one of the most in-depth ways of determining cellular gene expression levels of thousands of genes simultaneously. They are able to help determine: Getting good microarray results starts way before analyzing your data, however. A crucial first step in setting up a good microarray experiment is extracting high-quality RNA from your cells. In…
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One of my colleagues, a very good molecular biologist, told me that the only time she uses chemistry is when she needs to calculate molarities. I, of course, scoffed at this statement, and tried to remind her of all the chemistry she uses daily. True, I may be a bit biased since I am a…
Your DNA samples are precious so take care of them! Here are 5 ways that DNA can be damaged, so now you now what to avoid in the future.
Buying competent cells from commercial suppliers is convenient, provides a guarantee of quality, and gives access to strains with a variety of in-built traits that assist with things like maintenance of plasmid integrity (more on these traits later). However, this can be an expensive business. Alternatively, competent cells of any strain, including the specially-constructed commercial…
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