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Basically, you choose a gel based on two main factors: how high you need the resolution to be and what is in your samples. To make the choice simpler, I made a handy chart describing the differences between them.
*Pulsed field electrophoresis is a technique in which the direction of current flow in the electrophoresis chamber is periodically altered. This allows fractionation of pieces of DNA ranging from 50, 000-5 millon bp.
Use this chart to help you understand the differences between the two gel types and your samples will be running like athletes in no time!
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SMART Experiment Template
Want to make your next experiment diagnosable before you run it? The a one-page template fixes your objective, protocol, equipment, and reagent details in place before you run, so your workflow starts and stays reproducible.
This three-phase checklist covers everything you need to do before going home for the day. Cold storage, decontamination, and labelling will never get skipped in the rush again! Split responsibility clearly across first-in, personal, and last-out phases, each with its own sign-off.
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If you’ve worked in a lab, odds are you’ve had an encounter with filtration of one sort or another. Do you understand exactly how filters work, though? Or have you wondered why certain filters are used for certain lab applications?
Bubbles isn’t just the name of my favorite cartoon character from Power Puff girls, or just the best activity for a kid to play with, in general. In my adult world, they stand for a whole lot more, but can still cause extreme emotions. At the lab bench, seeing bubbles brings happiness or sadness depending…
When you work in a laboratory, preparing samples sets for many different experiments is a large part of the job. Keeping track of your samples can be tedious or even challenging if you don’t already have a good system in place. However, getting this right is a critical part of the experimental process. In this…
One of our readers posted the following question to us and we decided to pass it along to everybody’s favorite microbiology expert, Aunt Yersinia: For one year I am working in different research laboratories, after I got from school. I keep wondering why EVERYBODY is using pre-made Agar solutions for pouring plates, and EVERYBODY is…
Have you ever needed to work in a cold room for a long period of time? For example, if you need to dialyze or purify a protein of interest that is temperature sensitive, working in a 4°C cold room might be the only way to accomplish the work. Well, you are in luck. I dislike…
Designing and running PCR reactions in the lab has become so commonplace that the number of primer design tools available can be a bit overwhelming for a beginner (or even an experienced molecular biologist!). Below are four of my favorite online programs available to make primer design quick, easy, and effective. A quick note before…
10 Things Every Molecular Biologist Should Know
The eBook with top tips from our Researcher community.
