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Basically, you choose a gel based on two main factors: how high you need the resolution to be and what is in your samples. To make the choice simpler, I made a handy chart describing the differences between them.
*Pulsed field electrophoresis is a technique in which the direction of current flow in the electrophoresis chamber is periodically altered. This allows fractionation of pieces of DNA ranging from 50, 000-5 millon bp.
Use this chart to help you understand the differences between the two gel types and your samples will be running like athletes in no time!
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Guide to Lab Safety
This ebook introduces you to some of the most common safety principles that you will encounter every time you step into a laboratory. On top of that, it will help point you towards additional resources to answer your questions when deeper questions arise.
A neglected tool can easily play havoc with your experiments and ruin results. Download our handy poster to get top tips on proper pipette care. Stick it up in the lab to help remind yourself and any fellow lab mates how to look after this vital lab tool.
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One of my favorite things about being a biochemist is to imagine everything at the molecular level—sometimes, in very corny ways. I envision the proteins I pipet and mix as dynamic characters in a molecular soap opera that intermingle with each other in complex ways. The biomolecular characters in my soap opera interact and react,…
As a researcher, it’s satisfying to manage your own projects and do the bench work yourself. After all, if you don’t have experience with a technique, you’re usually expected to figure it out (with or without direct supervision). In some situations, dealing with difficult molecular techniques is simply part of the job description. The scientific…
When developing an assay, whether it is for basic research or for use in diagnostics, you will often be asked about your assay’s sensitivity. This is perhaps one of the most important performance characteristics you can determine for an assay, and in regulated work, such as in vitro diagnostic (IVD) development and clinical diagnostics, it…
Pop Quiz Time: You get a new bacterial strain from a culture collection, but you’re not quite sure what the genetic notation (i.e., all the letters and symbols) means. Do you: A. Cry?B. Ask around to see what your lab mates think?C. Cross your fingers that your friends at Bitesize Bio can help you out?…
Life in the lab is easy-peasy when you are only prepping a handful of tubes. But what if you need to scale up to 10’s or even 100’s of samples? Scaling up your experiments can have some expected and some not-so-expected, leaving you in a lurch, consequences. Read through our tips so you aren’t caught…
Ever re-run the same experiment three times just to keep getting “bad” results? In reality, no result is “bad” and experiments never “fail”. Instead, they produce data; sometimes that data is clear, sometimes it is not. After running an experiment, your results are either conclusive or inconclusive, and an inconclusive result is not useless. If…
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10 Things Every Molecular Biologist Should Know
The eBook with top tips from our Researcher community.
