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last updated: December 3, 2019
Rolly is Head of the Confocal and Advanced Light Microscopy (CALM) facility in the College for Medicine and Veterinary Medicine at the University of Edinburgh. In this function he also co-ordinates the strategic planning for various light microscopy-related projects across the College.
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It’s unclear exactly how the term ‘Special Stains’ first arose in the world of histology, but it refers to empirical and histochemical staining techniques that significantly contributed to the advancement of histology in the late 19th century. In a nutshell, these stains are ‘Special’ because they are not routine – simple as that. Therefore, Special…
Interested in the detailed structure of your tissue? High-resolution imaging techniques, such as brain electron microscopy, provide an intricate view of your tissue. While it may be a rather complicated procedure with nasty chemicals, the advantages of epon embedding can make it the best choice for morphological studies. The hard blocks are excellent for structural…
Although his name could fit in easily to the early 1980’s Hip-Hop Scene, Jerzy Nomarski (or ‘George’) was actually a Polish physicist with an interest in optical theory. Born in 1919, he eventually became a member of the Polish Resistance fighting in the Second World War. He was captured by enemy forces and held as…
Scanning electron microscopy (SEM) is a powerful technique, traditionally used for imaging the surface of cells, tissues and whole multicellular organisms (see An Introduction to Electron Microscopy for Biologists)(Fig. 1). While the resultant images appear to be three dimensional (3D), they actually contain no depth information. However, there are several SEM techniques that can obtain…
Do you use biotinylated secondary antibodies in your immunohistochemistry? You could use polymers instead. They are a great time-saving reagent.
Apoptosis, or programmed cell death, can be detected on tissue slides using stains in conjunction with immunohistochemistry and/or reporter assays.
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