Kathryn Lagrue

Articles by Kathryn Lagrue:

The Power of STED Microscopy Part 3: Dual Color STED

So far in this series I have given you an overview of how STED works and how to design your STED experiments. In this final article, I will tell you how to do two color STED and go over some tips and tricks to acquire the best images from your sample. Dual Color STED Fluorophore…

01 Jul 2015 Microscopy & Imaging

The Power of STED Microscopy, Part 2: Sample Preparation.

In part 1, “The Power of STED Microscopy: How Does it Work?” I covered the basics of STED Microscopy: how it works and why you might want to use it. If you now want to do STED Microscopy, the next step is to optimize your sample. In this article, I cover how you can get…

23 Apr 2015 Microscopy & Imaging

The power of STED microscopy, Part 1: How does it work?

Do you suspect that your favourite protein is doing something really cool? But you cannot see it because your confocal microscope’s resolution is limited. Then Stimulated Emission Depletion (STED) microscopy is what you need! With the power to smash through the diffraction limit of confocal microscopy, STED opens up a whole new world of improved…

18 Nov 2014 Microscopy & Imaging

Part 2: The Who’s Who of Super Resolution Microscopy – Single Molecule Localisation techniques

In part 1 of The Who’s Who of Super Resolution Microscopy I discussed one way to overcome the diffraction limit of light: ensemble technique. In this article I discuss another way to improve resolution: single particle localisation techniques. Single particle localisation techniques works by pin-pointing single molecules by reconstructing a super-resolution image from multiple frames…

21 Oct 2014 Microscopy & Imaging

The Who’s Who of Super Resolution Microscopy, Part 1

The ‘diffraction limit’ of a microscope is the minimum distance between two fluorophores where they can be still be discriminated as two separate objects. This diffraction limit has long constrained attempts by biologists to observe the intracellular environment. With a lower limit of ~200nm in confocal microscopy, this diffraction limit significantly limited the detail you…

12 Sep 2014 Microscopy & Imaging