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Kathryn Lagrue

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The Power of STED Microscopy Part 3: Dual Color STED

The Power of STED Microscopy Part 3: Dual Color STED

By Kathryn Lagrue | July 1, 2015

So far in this series I have given you an overview of how STED works and how to design your STED experiments. In this final article, I will tell you how to do two color STED and go over some tips and tricks to acquire the best images from your sample. Dual Color STED Fluorophore…

The Power of STED Microscopy, Part 2: Sample Preparation.

The Power of STED Microscopy, Part 2: Sample Preparation.

By Kathryn Lagrue | April 23, 2015

In part 1, “The Power of STED Microscopy: How Does it Work?” I covered the basics of STED Microscopy: how it works and why you might want to use it. If you now want to do STED Microscopy, the next step is to optimize your sample. In this article, I cover how you can get…

The power of STED microscopy, Part 1: How does it work?

The power of STED microscopy, Part 1: How does it work?

By Kathryn Lagrue | November 18, 2014

Do you suspect that your favourite protein is doing something really cool? But you cannot see it because your confocal microscope’s resolution is limited. Then Stimulated Emission Depletion (STED) microscopy is what you need! With the power to smash through the diffraction limit of confocal microscopy, STED opens up a whole new world of improved…

Part 2: The Who’s Who of Super Resolution Microscopy - Single Molecule Localisation techniques

Part 2: The Who’s Who of Super Resolution Microscopy – Single Molecule Localisation techniques

By Kathryn Lagrue | October 21, 2014

In part 1 of The Who’s Who of Super Resolution Microscopy I discussed one way to overcome the diffraction limit of light: ensemble technique. In this article I discuss another way to improve resolution: single particle localisation techniques. Single particle localisation techniques works by pin-pointing single molecules by reconstructing a super-resolution image from multiple frames…

The Who’s Who of Super Resolution Microscopy, Part 1

The Who’s Who of Super Resolution Microscopy, Part 1

By Kathryn Lagrue | September 12, 2014

The ‘diffraction limit’ of a microscope is the minimum distance between two fluorophores where they can be still be discriminated as two separate objects. This diffraction limit has long constrained attempts by biologists to observe the intracellular environment. With a lower limit of ~200nm in confocal microscopy, this diffraction limit significantly limited the detail you…

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