The protocol just deals with the bare bones of the digest, T4 DNA polymerase treatment, annealing and transformation. For information in designing the cloning site, see my original article, or the paper.
I previously wrote an article for BsB detailing my experience transitioning from lab-bench research into research administration roles after a particularly unhappy experience as a postdoc. About a year into my second research admin role some restructuring occurred and I decided to try to move back into the lab. I am now working again as […]
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