The protocol just deals with the bare bones of the digest, T4 DNA polymerase treatment, annealing and transformation. For information in designing the cloning site, see my original article, or the paper.
What is my most favorite task to do in the lab? Good question; it’s difficult to pick just one, so here are (quite) a few of my favorite things: 1. Working in a team This is what makes research great: in teams, numerous projects can be completed more quickly than what a single person may […]
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