The protocol just deals with the bare bones of the digest, T4 DNA polymerase treatment, annealing and transformation. For information in designing the cloning site, see my original article, or the paper.
As Morrissey once sang “in the midst of life we are in death, etc.” A fact of life in the research Lab is that whenever we run an assay there will almost certainly be some cell death in our sample. This may be insignificant in some techniques but in others it can be problematical. Why […]
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