The protocol just deals with the bare bones of the digest, T4 DNA polymerase treatment, annealing and transformation. For information in designing the cloning site, see my original article, or the paper.
Whenever you make up a solution or use a purchased reagent in the lab, you trust the work of a whole army of people and equipment. If any one of those links in the chain has a technical problem or makes a mistake, then your reagent might turn out to be faulty. And that can […]
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