The protocol just deals with the bare bones of the digest, T4 DNA polymerase treatment, annealing and transformation. For information in designing the cloning site, see my original article, or the paper.
According to the philosopher of science Thomas Kuhn, experimental science relies more on scientists’ emulation of each other as apposed to theoretical knowledge; e.g. it’s more like craft, which is transferred from person to person through teaching and observing, rather than anything else. Chosen by a group leader, a lab-management strategy is self-sustaining, so I […]
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