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Purified proteins must be kept soluble and active. Discover how to design the perfect protein purification buffer—from additives you can include and their appropriate concentrations to important considerations.
Ion-exchange chromatography is used to separate and purify proteins based on net charge at a particular pH. Here are the basics about this technique.
Using the right affinity tag can make protein purification a breeze. Read more to find out which tag is right for you.
We are all familiar with bacterial cell lines as a means for protein expression and purification. But can you do the same with eukaryotic cell lines? Read on to learn some helpful tips and considerations when needing to get your hands on some eukaryotic protein.
Purifying a new protein is no easy feat. Finding combinations of protein purification buffer, salt, detergent, and stabilizing agent to get high yields of squeaky-clean protein can become tedious. Few things are as bothersome during this process as Heat Shock Protein (HSP) contamination. But worry not, we’ve got some handy tips to avoid HSP contamination…
Is your goal to purify a substantial amount of a specific protein? Do you have a quantity of a molecule that binds your protein of interest? If so, generating a custom affinity matrix may be just the trick you need to purify your protein of interest by affinity chromatography. Customizing your affinity chromatography is an…
Perfect your subcellular fractionation in the lab by understanding how these protocols work. Plus, discover the various kits for organelle separation.
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