Ten Tips for Pipetting the 384-Well Plate

Ten Tips for Pipetting the 384-Well Plate

I was so excited to start using 384-well plates for my assays. With so many wells, these plates are useful for testing many conditions in parallel, as required in ELISAs, siRNA library screens, and drug treatment dilutions. However, I quickly learned that pipetting in these plates is more complicated than I thought. This article contains…

Top Tips to Avoid Multi-sample Labeling Chaos

Top Tips to Avoid Multi-sample Labeling Chaos

Imagine pipetting your publication experiment and then your favourite lab mate has an urgent question, which of course you helpfully answer. But when you finally turn back to your experiment you suddenly are not sure which pipetting step you were at. It’s happened to us all! Efficiently keeping track of your samples in a sometimes…

Control your error! How to minimize pipetting error and get low SDs in qRT-PCR

Control your error! How to minimize pipetting error and get low SDs in qRT-PCR

Variability is the Achilles’ heel of research. It can often confound our results and lead us astray searching for solutions. There are two kinds of variability, the first is biological variability. This represents the stochastic nature of the sample you are working with and the inherent differences between samples from the same conditions. There is…