RNA in situ hybridization - Human Melanoma FFPE Tissue Section (KRT5 and Housekeeping Gene)

Are You In(to) Situ? – Putting Together Your First RNAscope® Assay

ByChristina Lebonville

You are thinking of trying out RNAscope®. After all, RNAscope® holds promise for increasing the sensitivity and specificity of your in situ hybridization. Yet, getting started can be a little overwhelming with the numerous kits and reagents available in the RNAscope product line. Here’s an overview of your options to help you navigate to the…

FISHing for miRNAs in Archived Tissues? Yes, It Is Possible!

ByIlona Marecko

We use fluorescent in situ hybridization (FISH) techniques routinely to detect DNA or RNA sequences in tissues, but what about micro RNAs (miRNAs)? No worries, FISH is now optimized to meet the challenge. To help you get going with the method, here’s what you need to know. The first thing that comes to mind when…