“Viable But Non-Culturable (VBNC)”: Zombies of the Bacterial World

“Viable But Non-Culturable (VBNC)”: Zombies of the Bacterial World

Imagine that you want to test the efficiency of an antimicrobial treatment in inhibiting a certain bacterial pathogen. As part of the experiment, you expose the bacteria to the treatment and monitor the cultivability of the microorganism by counting the number of colony forming units (CFU) formed on culture media. If the microorganism is sensitive…

How to Properly Streak a Single Bacterial Colony

Bacteria are the workhorses of many molecular biology laboratories, and mastering the basic techniques to manipulate bacteria is an important stepping-stone towards achieving great results. When isolating DNA from bacteria, it is important to start with a single colony to ensure a homogenous population of bacteria in your culture. Isolating a single bacterial colony from…

Basic Bacterial Culturing Practices

Basic Bacterial Culturing Practices

Mastering basic bacterial culturing practices is a must if you are planning a career in microbiology! Growing bacteria might be one of the easiest things to do as a scientist. Also, as you’ve probably discovered, it’s even easier to do when you’re trying to prevent bacteria from growing where it shouldn’t be!! When we go…

Get Great Yields by Optimizing Your Bacterial Cultures

Get Great Yields by Optimizing Your Bacterial Cultures

Bacterial cultures may be much easier to grow than mammalian cells, but if your yields are suboptimal there are plenty of parameters to play with. Here we list a few of the things you should consider to maximize your culture growth. Shaking speed Shaking is performed to allow aeration of your culture, which is of…