unair
Usha Nair holds a PhD in structural biology from Jawaharlal Nehru University, New Delhi, India and has several years of postdoctoral experience in protein expression and purification and their biochemical and biophysical characterization. At the moment, she is trying to find funding for developing novel in vitro methods for the expression of biologics and biosimilars.
Articles by unair:
Using a Gel Filtration Chromatogram to Estimate Molecular Weight
Gel filtration chromatography (also known as size exclusion chromatography, molecular sieve chromatography, or gel permeation chromatography) is based on the differential distribution of the components in a sample between the mobile and stationary phases. Specifically, in gel filtration chromatography, this differential distribution depends on the size and shape of the components. Here, we take a…
The Real-Time PCR Digest
In the 30 odd years since its invention, the polymerase chain reaction (PCR) has become the bread and butter technique of molecular biologists. The secret to its indispensability lies in its simplicity and versatility. Numerous variants of the technique have been developed; one of these, real-time PCR, has become the method of choice for quantitative…
Gel Filtration Chromatography Media: What You Need to Know
Do you use pre-packed Superdex 75 or Superdex 200 columns for gel filtration chromatography? Ever wonder if other media could better address your purification needs? Look no further. Your quest ends here! But if you’re looking for information on gel filtration principles, check out this earlier article. Choosing a Column for your Gel Filtration Experiment…