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Dr. Omonse Talton

Omonse is a post-doctoral fellow at the University of Missouri where she studies developmental programming (look up DoHAD) and teaches Cell Biology.

Articles by Dr. Omonse Talton:

Assays: Wellular to Cellular

Out with the Old… Well-based assays have been the standard for common laboratory experiments, such as fluorescence cytometry. A researcher places a small amount of sample into a well on a plate and assays it, which produces a single data point. However, this so-called single data point is actually an average of the measurements of…

02 Aug 2019 Cells and Model Organisms&Tecan

10 Tips on Mating Mice Successfully

Tiny, furry, spinning around a wheel – few creatures are as endearing as the lab mouse. Trying to obtain reproductive success with them, however, can leave you spinning your own wheels. Why is it that what works so well for the animal facility staff, or experienced technician, seems to be beyond your reach? After all,…

28 Feb 2019 Cells and Model Organisms

Lab Hacks: Tips to Save You Money and Time

Not every lab has the cash to shell out on fancy equipment. And let’s face it, in today’s climate of budget cuts, even the baller labs are looking for ways to be more frugal. Years of lab work has taught me a thing or two on how to make ends meet. So, if you’ve found…

21 Sep 2018 Equipment Mastery and Hacks

Primer Validation Was a No-Go – Now What?

The primers for your gene of interest have finally arrived in the mail, and you’re ready to figure out whether your favorite gene’s expression level is elevated in those precious tissue samples. Only one small last step before you can proceed. Primer validation. This is a standard procedure where you run PCR or qPCR on…

13 Mar 2018 PCR, qPCR and qRT-PCR&Qiagen

Troubleshooting a Faulty ELISA

Is ELISA giving you the blues? The frustrating kind, not the lovely kind you get while watching the enzyme substrate reaction! This age old assay has the perks of being quick and fairly simple to perform, but when conditions are not perfect, ELISAs can deliver less than optimal results, and fail to be consistent and…

14 Feb 2018 Protein Expression and Analysis