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Dr. Omonse Talton

Omonse is a post-doctoral fellow at the University of Missouri where she studies developmental programming (look up DoHAD) and teaches Cell Biology.

Articles by Dr. Omonse Talton:

Assays: Wellular to Cellular

Out with the Old… Well-based assays have been the standard for common laboratory experiments, such as fluorescence cytometry. A researcher places a small amount of sample into a well on a plate and assays it, which produces a single data point. However, this so-called single data point is actually an average of the measurements of…

02 Aug 2019 Cells and Model Organisms&Tecan

10 Tips on Mating Mice Successfully

Tiny, furry, spinning around a wheel – few creatures are as endearing as the lab mouse. Trying to obtain reproductive success with them, however, can leave you spinning your own wheels. Why is it that what works so well for the animal facility staff, or experienced technician, seems to be beyond your reach? After all,…

28 Feb 2019 Cells and Model Organisms

Lab Hacks: Tips to Save You Money and Time

Not every lab has the cash to shell out on fancy equipment. And let’s face it, in today’s climate of budget cuts, even the baller labs are looking for ways to be more frugal. Years of lab work has taught me a thing or two on how to make ends meet. So, if you’ve found…

21 Sep 2018 Equipment Mastery and Hacks

Primer Validation Was a No-Go – Now What?

The primers for your gene of interest have finally arrived in the mail, and you’re ready to figure out whether your favorite gene’s expression level is elevated in those precious tissue samples. Only one small last step before you can proceed. Primer validation. This is a standard procedure where you run PCR or qPCR on…

13 Mar 2018 PCR, qPCR and qRT-PCR

Troubleshooting a Faulty ELISA

Is ELISA giving you the blues? The frustrating kind, not the lovely kind you get while watching the enzyme substrate reaction! This age old assay has the perks of being quick and fairly simple to perform, but when conditions are not perfect, ELISAs can deliver less than optimal results, and fail to be consistent and…

14 Feb 2018 Protein Expression and Analysis

Having a Family in Grad School

The question that many graduate students have is “can I have it all?” A successful, productive PhD and marriage? And kids too? There is no doubt that having a family in grad school takes away your focus for a while especially around the time your baby is born, but it can also increase your productivity.…

16 Aug 2017 Organization and Productivity

The Dumbed-Down Thesis: A Little Reddit Fun

Science talk can be cryptic, and scientists are realizing that nobody knows what the heck they’re talking about half of the time. To combat this, science communication lobbyists have done a great job of introducing new ways to communicate science that don’t involve babbling on and on. Have you ever practiced a 30 Second Elevator…

02 Jan 2017 Survive and Thrive

How to Keep Your Centrifuge Alive

According to the Occupational Safety and Health Administration, “the majority of all centrifuge accidents result from user error.” That’s right, it’s us. Many a machine has been destroyed by an unsuspecting scientist just in it for the pellet. The more expensive the machine, the more sensitive and the easier it is to break. Does your…

23 Nov 2016 Equipment Mastery and Hacks

How do you Solve a Problem like Pseudoscience?

If you need hair advice are you more likely to scour the Trichological Society’s website, or head over to Pinterest? In the age of the search button, people have access to all sorts of “information,” and knowledge is power. No subject or discipline is shielded from social media, and now more than ever the public…

10 Aug 2016 Science Communication & Ethics

Aliens from Outerspace: Navigating a Lab in the West

If you find it very difficult to call your boss by her first name, feel obligated to wear business casual clothes to the lab, or reckon that it’s slightly weird that your fellow PhD candidates are inviting you to a casual night out instead of to the library to study, then like me, you just…

09 Jul 2016 Survive and Thrive

4 Tips for Extracting RNA from Unfriendly Tissues

Some tissues are tricky to work with. This truth was lost on me in the early years of grad school because I worked with liver samples. If you’re extracting RNA from liver samples, you’re likely not losing sleep over your massive RNA yields. But for the folks doing RNA extractions with less willing donors, such…

09 Jul 2016 DNA / RNA Manipulation and Analysis