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Lesley McCollum

Lesley obtained a PhD in Neuroscience from the University of Alabama at Birmingham, where she studied the neurobiology of schizophrenia.

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Articles by Lesley McCollum

All You Need to Know (and More) About Embedding for Electron Microscopy

By Lesley McCollum | June 28, 2017

Interested in the detailed structure of your tissue? High-resolution imaging techniques, such as brain electron microscopy, provide an intricate view of your tissue. While it may be a rather complicated procedure with nasty chemicals, the advantages of epon embedding can make it the best choice for morphological studies. The hard blocks are excellent for structural…

tissue embedding

Tissue Embedding Throwdown: Paraffin vs OCT vs Resin

By Lesley McCollum | June 28, 2017

Tissue embedding and sectioning is a backbone of many biological research labs. While commiserating with other grad students over tedious hours spent in the lab, you’re probably aware that there is more than one way to slice up a chunk of tissue. We’ve previously introduced what to consider when choosing a tissue embedding medium and…

Unpacking the Daunting Task of Stereology for Electron Microscopy

By Lesley McCollum | June 7, 2017

Unpacking the Daunting Task of Stereology for Electron Microscopy Electron microscopy provides fantastic detail and resolution, with brain electron microscopy allowing visualisation of neurons and their individual synaptic connections. You may find yourself needing to count these neurons or connections, which can easily go into the billions. But counting these one-by-one isn’t really feasible. This…

human tissue

The Messy World of Human Tissue Research

By Lesley McCollum | August 31, 2016

In neuroscience and other biomedical research fields, animal models allow us to answer critical but otherwise impossible questions. Despite the value of these models, however, sometimes nothing can replace the real deal—human tissue. The limitations of human tissue research stem from the variability between subjects and the risk for covariates that influence your results. Which…

How to Take a Publication-Worthy Electron Micrograph

How to Take a Publication-Worthy Electron Micrograph

By Lesley McCollum | July 9, 2016

It doesn’t matter how excited you are about the research or how intriguing the biology is, if you cannot record it – and record it well – it won’t matter. Here I will tell you how to take the perfect electron micrograph.

free-floating or slide-mounted

Free-Floating Versus Slide-Mounted Sections for Immunohistochemistry

By Lesley McCollum | July 9, 2016

After countless immunos with free-floating sections – troubleshooting, testing antibodies, and finally doing the actual experiments – I felt like an expert on immunohistochemistry. I knew everything there is to know, right? Well, of course not – it does not work like this in science! For my next project, I would need to perform immunohistochemistry…

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