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Jason has a BS in biochemistry, runs experiments in a behavioral neuroscience laboratory at Oregon Health & Science University, and creates custom tools to increase the speed and efficiency at which the building blocks of your scientific discoveries can be made.
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Coating (or ‘subbing’) slides for immunohistochemistry can be the difference between having an organized set of tissue slices ready for microscopy- or watching them detach and float away during a wash. It takes a lot of time to place tissue slices in correct anatomical order, aligned right-side up and flat. To the naked eye, all…
Discover the different approaches to FRET quantification and get tips for selecting your FRET pairs.
You may already use fluorescence as a tool in your microscopy and imaging work, but, do you know exactly what it is? Why are certain proteins and probes fluorescent? What causes this light emitting property? We’ll have a look at these and more questions in this article. Start with a definition We’ll start with a…
Don’t be daunted by imaging and analyzing your wound healing assay; our top tips will get it looking perfect.
In my previous article I covered different immunohistochemical staining techniques at a superficial level. In the following articles I will start to explain these technologies in a bit more detail and in which situations they should be applied. All of the following will involve additional stages when applying them, for example- serum blocking, protein blocking,…
Purchasing a microscope camera is one of the most daunting tasks you might have to undertake. Before you set out to buy that camera, carefully consider your applications. Things like sample brightness or the speed of the phenomenon you are trying to capture can dictate your choices. Also, this is the time to make peace…
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