Suganth Kannan's Profile

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Demystifying REBASE and NEBcutter

Two years ago, all I knew was third BASE in my baseball field and the cutter ball from the pitcher. Now, I know a lot more about lab-based BASES and cutters: REBASE and NEBcutter. While they sound like baseball terms, REBASE and NEBcutter are tools for working with restriction enzymes. Read on to find out […]

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In DNA / RNA Manipulation and Analysis 12th of September, 2016
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Are You Ready for Your First qPCR?

Hope you had a heavy breakfast, because the first qPCR is going to take time. Did you remember to fill your coffee cup? There are a lot of intricacies in qPCR that will need your neural networks to be brisk. How qPCR differs from traditional PCR Unlike traditional PCR, qPCR measures the amplification of DNA […]

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In PCR, qPCR and qRT-PCR 9th of July, 2016
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4 Easy Steps to Analyze Your qPCR Data Using Double Delta Ct Analysis

You are at the airport burning away time with a report due tomorrow morning for your professor. You have your data. Why not take advantage of the time and calculate the expression fold change for the gene you have tested in the lab? It’s easy – I’ll show you how. What you need for analysis […]

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In PCR, qPCR and qRT-PCR 9th of July, 2016
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Six Facts About Restriction Enzymes

When restrictions come in the form of paperwork and approvals, we detest them. Whereas, when the restrictions come in the form of enzymes, we love them, don’t we? Restriction enzymes play a key role in biotechnology research. Read ahead for six useful facts about restriction enzymes.  1.  Restriction enzymes are helpful to bacteria Restriction enzymes […]

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