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Library Preparation read on

Improving Library Preparation for Next Generation Sequencing

Library quantity and quality are paramount to the success of a Next Generation Sequencing (NGS) experiment. But there are a lot of factors that impact library yield and quality: conversion rate, GC coverage, low quality or low quantity input material, and PCR-introduced bias. All that, and wouldn’t a shorter protocol be nice too? Whether you […]

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Simple Tips to Improve your Cloning Efficiency

Since the early 1970s, molecular cloning has used restriction enzymes to excise fragments from source DNA and to linearize plasmid vectors. After purifying the insert and vector, and ensuring compatible ends, both are joined with the help of a DNA ligase. The resulting recombinant vector is then used to transform an E. coli host into […]

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