On the contrary, just like everything else in research, SDS-PAGE can go wrong in a multitude of ways. And if you took a peek inside some of my old lab books you would have all of the proof you need about how easily this technique can make you look like a fool.
But luckily, I don’t have to air any dirty laundry in public to show you, because someone else has done something similar already.
As part of a lab guide for an experimental biology course at Rice University, David R. Caprette has pulled together an “SDS-PAGE Hall of Shame“. It’s made up of photos of gels produced by course students, as well as some from the university’s research labs, that have gone horribly wrong for all manner of reasons.
As well as providing entertainment for ghoulish (science geek) onlookers, the gallery is, of course for educational purposes. It is intended as a troubleshooting resource; by clicking on the picture that looks most like your poor, messed up gel you will be given a pearl of wisdom that suggests what you might have done wrong so you can remedy it in the future.
But perhaps the best things about it is that in an uncertain world, it shows that everyone else has protein gel disasters too. And most of us can probably take comfort from that.
There are so may statistical tests out there it can be difficult to determine which is the right test to use. Below is a simple diagram to help you quickly determine which test is right for you. Although this is by no means a comprehensive guide, it includes some of the most common tests and […]
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