On the contrary, just like everything else in research, SDS-PAGE can go wrong in a multitude of ways. And if you took a peek inside some of my old lab books you would have all of the proof you need about how easily this technique can make you look like a fool.
But luckily, I don’t have to air any dirty laundry in public to show you, because someone else has done something similar already.
As part of a lab guide for an experimental biology course at Rice University, David R. Caprette has pulled together an “SDS-PAGE Hall of Shame“. It’s made up of photos of gels produced by course students, as well as some from the university’s research labs, that have gone horribly wrong for all manner of reasons.
As well as providing entertainment for ghoulish (science geek) onlookers, the gallery is, of course for educational purposes. It is intended as a troubleshooting resource; by clicking on the picture that looks most like your poor, messed up gel you will be given a pearl of wisdom that suggests what you might have done wrong so you can remedy it in the future.
But perhaps the best things about it is that in an uncertain world, it shows that everyone else has protein gel disasters too. And most of us can probably take comfort from that.
RNA interference (RNAi) may have originated as a defense mechanism to protect cells against foreign genes introduced by viruses. This concept has since been put to use to create a powerful experimental tool for investigating gene function in organisms. Small-interfering RNA (siRNA) libraries for investigating genome-wide function can be produced by chemical synthesis of probes […]
It’s great to have you in the Bitesize Bio family! We’ve sent you an email to confirm your registration. Please click on the link in the email or paste it into your browser to finalize your registration.
For more information on how to use Bitesize Bio, take a look at the following image (click it, for a larger version)
An error occured while registering you, please reload the page and try again