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Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
So, you’ve designed PCR primers to amplify your sequence of interest, and you’re ready to go. But unless you have a never-ending supply of template, polymerase, and a thermocycler with a gradient function—not to mention a hefty dose of time and patience—you probably don’t want to spend the next week finding the perfect conditions for…
The eBook with top tips from our Researcher community.