The Correct Way To Quantify Cellular Autophagy

The Correct Way To Quantify Cellular Autophagy

Just like you need to clean up your room from time to time, your cells also need to do a bit of housekeeping.  Your cells accomplish this through a process called autophagy.  Autophagy mainly serves two roles.  The first is to remove damaging materials, such as misfolded proteins, dysfunctional organelles, and foreign invaders.  The second…

An Experimental Tool-kit for Measuring Protein Stability

An Experimental Tool-kit for Measuring Protein Stability

Proteins in the cell are in a constant flux governed by events including synthesis and degradation. In an effort to make cells more efficient by reducing the unnecessary protein load, most proteins in the cell have a specifically defined half-life. Another reason why cells have evolved to degrade proteins is to ensure timely removal of…

How to Store Your Concentrated Proteins

Like graduate students, proteins are sensitive to rough handling. This is particularly true when they (the proteins, not the students!) are being concentrated, purified, and stored. We’ve covered the many options out there for concentrating your proteins, along with how to handle protein extracts to keep your proteins safe from degradation. But proteins can degrade…