Cut My Gene into Pieces– Introduction to Restriction Enzyme Cloning

Cut My Gene into Pieces– Introduction to Restriction Enzyme Cloning

At the heart of cloning are restriction enzymes. Restriction enzymes are a common tool in any molecular biology lab. Need to know how large your plasmid is? Cut it with a restriction enzyme. Need to chop your genomic DNA into smaller pieces for a southern hybridization or to prepare a library? Use a restriction enzyme….