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Seeing Potential: How to Visualise Your Multipotent Cells

The cells I work with are special — they’re stem cells. This means that when it comes to deciding their ultimate fate, they’re still sitting on the fence. This ability is great for identifying pathways of cellular development and differentiation. It also serves research into tissue regeneration and human disease. But how can we make…

From Cells to Scope: Chamber Slide Immunochemistry

Immunolabeling is the tried-and-true immunochemistry method of getting the stain you want onto the molecular target you want. Whether that target is contained within a large region of tissue (immunohistochemistry) or inside a single cell (immunocytochemistry), the ability to accurately label large numbers of samples will simplify your workflow and help you to achieve excellent…

Gateway to the Cellular Kingdom: Cell Fixation and Permeabilization Techniques

One of the much sought after question asked by many researchers worldwide is – “What is the gene expression profile of a single cell within a heterogenous pool of cells?” While mass cytometry is the current ‘hot’ methodology for single cell analysis, the good old flow cytometry can help us perform rapid analysis of single…