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Autofluorescence, background fluorescence in unstained cells or tissues, often interferes with microscopy clarity. The article outlines causes, such as natural fluorophores like NADH, and offers strategies like selecting distinct fluorophores and optimizing sample prep to minimize its impact and enhance image accuracy.
Selecting fluorophores can be a tricky business, but we’ve got you covered in this handy how-to guide.
So far in this series I have given you an overview of how STED works and how to design your STED experiments. In this final article, I will tell you how to do two color STED and go over some tips and tricks to acquire the best images from your sample. Dual Color STED Fluorophore…
A Brief History of Detecting Amplicons The Old Days of Ethidium Bromide In the early 1990s, quantitative (q) PCR was in its infancy, and despite PCR itself already being around for 10 years, there were no easy ways of precisely quantifying the amount of DNA that was amplified in a PCR reaction. In those days PCR…
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