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Electroporation

Women holding a broken electricity cable looking like she's been electrocuted to represent the competency of electrocompetent E. coli
Protein Expression and Analysis

DIY Electrocompetent E. coli

ByDr Nick Oswald

Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.

Read More DIY Electrocompetent E. coliContinue

How to Transform Microalgae
Cells and Model Organisms

How to Transform Microalgae

ByDaphne Ng

What is the first image which comes to mind when you think about microalgae? Green scum that covers the surfaces of ponds? Unsightly stains on pavements and walls? Far from being a nuisance in ponds, lakes, drains and on surfaces, microalgae are fascinating microorganisms which are used to understand various biological processes. Microalgae have been…

Read More How to Transform MicroalgaeContinue

Transfection Toolkit
DNA / RNA Manipulation and Analysis

Transfection Toolkit

ByChelsey Kline

Engineering a mutation or overexpressing a recombinant protein to study and characterize its function in mammalian cells is no easy task. Luckily, Chinese hamster ovary (CHO) cells, which have been a mainstay in the lab since the 1950s, represent a relatively easy mammalian model system to engineer. There are several methods to choose choose from…

Read More Transfection ToolkitContinue

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