Delta Delta Ct

Scientist loading PCR tubes into a PCR machine to represent Ct values in PCR testing.

PCR, qPCR and qRT-PCR

What Is a Cq (Ct) Value?

ByDr Nick Oswald October 27, 2023July 23, 2024

Don’t be confused by Ct values! We’ll guide you through what they are, how to calculate them, and troubleshooting issues.

Fingers "walking" up wooden blocks arranged as steps, representing the steps in Ct values from a qPCR experiment, analysed by double delta Ct analysis

PCR, qPCR and qRT-PCR

4 Easy Steps to Analyze Your qPCR Data Using Double Delta Ct Analysis

BySuganth Kannan March 11, 2021June 17, 2023

New to qPCR? Here’s a quick summary of one of the two most common analysis methods – double delta Ct analysis.