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Achieving publication-quality immunofluorescence images is tricky. Learn what controls for immunofluorescence you can use to get them!
What is one of the first steps of the flow cytometer start-up routine? To check if the cytometer is in good shape, of course! Everyone wants to run on a reliable instrument, and assessing the daily performance of the instrument show us its behaviour over time and allows us to react fast in case of…
Excellent research takes time and effort, and a publication is your chance to showcase your hard work. While your main motivation might be to share and discuss your results, your methodology section is key to the reproducibility of your work, acting as a foundation for other researchers to repeat and build upon your findings. In…
As scientists, we are trained to design an experiment with the bigger picture in mind; the ultimate goals being to publish quality data and demonstrate scientific rigor. However, sometimes you need to focus on the little things, such as perfecting control and experimental samples, incubation times, and ordering reagents to truly ensure that you obtain…
For many students, a PhD project is the first opportunity to really sink your teeth into your very own research project over a long period of time. This initial period is exciting but can also be a little daunting. Where do you begin? How do you actually design your first experiments? I mean, what are…
In many biological experiments the question that a researcher wants to ask is – ‘do some or all of my cells express a particular protein?’ There are many ways of doing this, which you will be familiar with e.g. Western blotting, immunoprecipitation, microscopic examination of stained cells and even mass spectrometry. Using Flow Cytometry to…
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