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After a late night transformation you realise you have forgotten to make any plates. Should you use the old stash of amp plates you found in the back of the cold room?
I often wonder why it is that molecular biology researchers stubbornly refuse to change 40-year old methods that, while work, are not as good as newer, faster and cheaper methods out there. I suppose rational scientists often have irrational superstitions. One example of an old method that could be improved is the growth media used…
There’s more than one way to do a plasmid miniprep. Here are 5 to add to your molecular biology arsenal.
You know those ridiculously priced and throw-away DNA mini, midi and maxi-prep columns? Well the good news is that you can actually re-use them if you are reasonably careful at regenerating them, with this simple and cheap method described in detail by Nagadenahalli B. Siddappa in Biotechniques in 2007. Apparently these columns can be reused…
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