Techniques / PCR, qPCR and qRT-PCR

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How to Choose the Correct Reverse Transcription Method

Quantitative Reverse transcription PCR (RT-qPCR) is frequently used in the lab to detect and quantify RNA expression in a sample. The first step of the assay is to convert the labile RNA to its complementary DNA (cDNA) counterpart through reverse transcription (RT). In fact, RT is the first step in a variety of molecular biology […]

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Decoding Cancer: Practical Advice for Working with Cellular Heterogeneity

In this webinar, you will learn about the process for genotyping cells—particularly tumor cells—and practical advice for analyzing the resulting data. The main points that we will cover are: Practical advice and methods for sample collection and preparation for your genotyping experiments An overview of the available data analysis methods for your genotyping results Examples […]

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In PCR, qPCR and qRT-PCR, Qiagen 9th of August, 2016 Presented by: Ania Wronski
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Quenching to Success with BHQnova Probes: Turn to the Dark Side

The ability to specifically detect and quantify the amount of a particular nucleic acid sequence in a sample has revolutionized molecular biology and science as a whole. We can now elucidate many genetic elements, such as identify genomic copy number variations, the presence of pathogens, or assess the expression of a gene in large populations. […]

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What Is a Ct Value?

When conducting real-time PCR, you’re looking for the exact amount of a target sequence or gene in your sample. During the PCR reaction, you measure its progress by accumulation of a fluorescent signal during amplification. But there’s also a lot of background fluorescence, which you want to bypass in order to glean meaningful information from […]

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In PCR, qPCR and qRT-PCR 16th of July, 2015