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yeast two hybrid

Image of two hands shaking to represent the physcial interaction of proteins in a quantitative yeast two-hybrid assay
Protein Expression and Analysis

A Step-by-Step Guide to the Quantitative Beta-galactosidase Assay for Y2H Screening

BySafia Mahabub Sauty

Want to know not only if your proteins bind but quantify the interaction? We walk you through how to perform a quantitative yeast two-hybrid assay.

Read More A Step-by-Step Guide to the Quantitative Beta-galactosidase Assay for Y2H ScreeningContinue

Split Ubiquitin Yeast Two-Hybrid
Protein Expression and Analysis

Split Ubiquitin Yeast Two-Hybrid

ByMichelle van Geldermalsen

If you’ve read our article, An Overview of Yeast Two-Hybrid (Y2H) Screening, you’ll know that one major limitation of conventional Y2H is that your protein-protein interaction must occur in the nucleus for the reporter gene to be activated. So what do you do if your protein is a receptor tyrosine kinase? Or a G protein–coupled…

Read More Split Ubiquitin Yeast Two-HybridContinue

An Overview of Yeast Two-Hybrid (Y2H) Screening
Protein Expression and Analysis

An Overview of Yeast Two-Hybrid (Y2H) Screening

ByMichelle van Geldermalsen

If you are cooking up a way to test if two proteins interact, you need a yeast two-hybrid (Y2H) screen in your recipe book. You don’t want your Y2H to turn out half-baked – so check out this guide to Y2H and we’ll help you make sure yours will rise to the occasion! What is…

Read More An Overview of Yeast Two-Hybrid (Y2H) ScreeningContinue

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