How Good Is Your Best Friend? A.K.A. Have You Validated Your Antibody?

How Good Is Your Best Friend? A.K.A. Have You Validated Your Antibody?

You use your antibody frequently, maybe even every day. You rely on it for western blotting, immunohistochemistry, FACS, ELISA, and immunoprecipitation. You’d be lost without it. But how well do you really know your antibody? Are you sure that it detects what you believe it to detect? If you have the slightest doubt, or if you have…