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SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Reproducibility is a cornerstone of scientific research and your results need to be reproducible not only by yourself but also by others, both in and outside of your laboratory. This reproducibility is key for validation of your results as well as to further expand on the knowledge gained during the experiment. In order to accurately…
We’ve all been there. You’re looking to replicate a result you have read in a paper, or maybe even one that has come from someone else in your own lab. But try as you might, you can’t get your head around the less than effective lab protocol that’s been provided. Or, worse still, you are…
Some techniques can sound very dry but this isn’t one of them! SAGE was first described and published by Velculescu et al. in 1995. At the time, techniques like RNA blotting and expressed sequence tagging were used to study gene expression. However techniques like these were slow and very limited. The speed of SAGE and…
One of the most exciting things about being a scientist is doing experiments that have never been done before. Unfortunately, this can also be one of the most daunting aspects of science. Read on to make taking on your next experiment less daunting.
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