In Situ Zymography: Let’s Catch that Enzyme in Action!
Discover a way to study the active form of proteases in situ with in situ zymography. Read this article to get up to speed.
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Discover a way to study the active form of proteases in situ with in situ zymography. Read this article to get up to speed.
Every biochemist is familiar with proteases. More often than not, proteases cause a lot of anxiety. To this end, a lot of research has been done in developing techniques to prevent the activity of proteases. But some of these proteases can be the good guys too! For example, you can use them to separate your…
In this article I will not talk about ‘wild’ proteases, which destroy cellular proteins in your lysates like wolves destroy sheep. Instead, I’ll be talking about the shepherd dog proteases—purified, tame and useful to digest proteins your research. In Protein Research and Crystallization Several programs can predict your protein domains. However, we wet biologists know…
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