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qPCR primer design is a bit of science, a bit of magic, and a little bit of luck. Here’s the science to help you design the best primers for your experiments.
How to Prevent False Results in Colony PCR Colony PCR saves time and reduces costs by eliminating the need for plasmid purification. However, confounding results abound — but only if you fail to anticipate them. This article outlines the major perpetrators of false results and how to prevent them. For a more general overview of…
I am sure many of you have been there. Everything is going smoothly, and your project seems to be working out perfectly. And then there is this one PCR. For some reason, it just won’t work. It is a black dot on your record. Even though I have a scientific mind, I have to be…
Designing effective qpcr primers is essential for successful qPCR experiments, yet it can be challenging. Many validated primers are available through commercial databases like Qiagen’s GeneGlobe and Applied Biosystems’ TaqMan assays. Alternative public databases also offer primers, mainly for human and mouse genes. When primers are unavailable, literature searches or custom design remain necessary. Using pre-designed qpcr primers can save time and improve assay efficiency, making them valuable resources for bioscience researchers.
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