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Pipetting errors getting you down? Learn how to avoid and correct errors that hurt your pipetting accuracy in this easy-to-follow article.
Pipetting all day? Scrolling and scrolling through Excel columns trying to make sense of your data? Spending hours at the microscope because your boss wants Nature-worthy pictures? It’s not uncommon that performing lab work forces you into unhealthy postures, and after a day at work your spine begs for mercy. How Your Posture Suffers on…
I was so excited to start using 384-well plates for my assays. With so many wells, these plates are useful for testing many conditions in parallel, as required in ELISAs, siRNA library screens, and drug treatment dilutions. However, I quickly learned that pipetting in these plates is more complicated than I thought. This article contains…
This article explains how to use Microsoft Excel to simplify qPCR Plate Design, reducing errors and confusion when setting up multi-well plates. By creating detailed plate maps and using Excel’s speak upon enter feature, researchers can accurately add variable RNA volumes and reagents, improving efficiency and confidence in complex qPCR workflows.
Imagine pipetting your publication experiment and then your favourite lab mate has an urgent question, which of course you helpfully answer. But when you finally turn back to your experiment you suddenly are not sure which pipetting step you were at. It’s happened to us all! Efficiently keeping track of your samples in a sometimes…
Minimizing pipetting errors in qPCR is essential for reducing technical variability in qRT-PCR experiments. Key strategies include maintaining focus to avoid distractions, using a consistent pipetting order, preparing master mixes to increase volume accuracy, running multiple replicates, and regularly calibrating pipettes. Employing multichannel pipettes and understanding reagent properties also improve precision. These practices help generate reliable, low standard deviation data critical for accurate gene expression analysis.
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