Titering Phage – Counting Something Invisible with Something Only Slightly More Visible

ByBrian McWilliams

Titering Phage – The Plaque Assay Phage display is a molecular technique used to isolate binding or interaction partners to molecules of interest from an extensive library. Such libraries are often derived from the variable regions of native B-cell antibody-binding genes cloned into phage DNA. A single round of phage display panning involves many important steps. However, the…

Finding a Needle in a Needlestack Using Phage Display

Finding a Needle in a Needlestack Using Phage Display

ByBrian McWilliams

Few things can dash your hopes quite like phages. They can annihilate whole bacteria cultures in the blink of an eye, and make your next cloning or expression project impossible. But you can harness these evil-do-ers for good. And use phages to screen massive libraries of peptides. Learn how below. The Typical “Evil” Phage Experience…

11 Reasons Why You Should Use Recombinant Antibodies (rAbs)

11 Reasons Why You Should Use Recombinant Antibodies (rAbs)

ByDaad Abighanem

Monoclonal antibodies: You’ve probably heard a lot about them. Unsurprisingly, you may have also used them in your research. These antibodies (mAbs) are classically produced by the hybridoma technology pioneered by Köhler and Milstein in 19751: A mouse is immunized with the substance against which you need to produce an antibody. The mouse spleen cells (consisting…