Quantitative RT-PCR: One-step or Two-step RT?
Need to choose between one-step and two-step RT-qPCR? If so, you’ll want to get it right the first time. If you’re not sure where to start, this article outlines a decision framework you can use, including an interactive tool that gives you a recommendation based on your specific experiment. One-step RT-qPCR combines reverse transcription and…

