The Importance of Non-coding RNAs
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The Importance of Non-coding RNAs

What Are Non-Coding RNAs? What was once considered “junk” may end up being the most important part of our genome. Non-coding RNA (ncRNA) is RNA that is transcribed from DNA but diverts from the “central dogma” because it does not code for proteins. NcRNAs are ubiquitous in eukaryotes: while 90 percent of eukaryotic genomes are…

FISHing for miRNAs in Archived Tissues? Yes, It Is Possible!

We use fluorescent in situ hybridization (FISH) techniques routinely to detect DNA or RNA sequences in tissues, but what about micro RNAs (miRNAs)? No worries, FISH is now optimized to meet the challenge. To help you get going with the method, here’s what you need to know. The first thing that comes to mind when…

From Revolution to Evolution: Stem-loop Real-time PCR

From Revolution to Evolution: Stem-loop Real-time PCR

Kary Mullis invented polymerase chain reaction (PCR) in 1985 creating a revolution in molecular biology techniques. But it hasn’t stopped there. PCR has greatly evolved over the years. Today, we stand at a point, where we can clone micro RNAs (miRNAs) in real time! Due to miRNA size (about 18-21 nucleotides long) and varied expression levels,…

Mysterious miRNA: Identifying miRNAs and Their Targets

Mysterious miRNA: Identifying miRNAs and Their Targets

In my first article on this topic we delved into what miRNAs are, how they are generated, and their function. Now, we are going to discuss how to identify miRNAs and their targets. Why Do You Want to Look at Something So Small Anyhow? miRNAs play a crucial role in most physiological processes. It’s not…

Mysterious Plant miRNAs: What About Them?

Mysterious Plant miRNAs: What About Them?

Welcome to the last article in this series! Last, but by no means least, we will look at the importance of plant miRNAs and how they differ from their animal counterparts. When/How Were Plant miRNAs Discovered? Plant miRNAs were first described in 2002, a decade after the seminal miRNA study in the nematode C. elegans…