Decisions, Decisions: How to Choose the Best qPCR Probe for Your Experiment

Decisions, Decisions: How to Choose the Best qPCR Probe for Your Experiment

Before we go any further, we have to get some things straightened out: RT-PCR versus qPCR versus RT-qPCR. Sooo confusing, amirite?? They all refer to specific molecular biology assays, but the names are unfortunately used interchangeably, which can be awfully confusing for just about anyone. So without further ado: RT-PCR is short for reverse-transcriptase PCR,…