8 Basic Points to Remember Before Expressing Proteins in Bacterial Systems

8 Basic Points to Remember Before Expressing Proteins in Bacterial Systems

As a protein biochemist and a Ph.D. student, I was given the task to express a eukaryotic protein in a bacterial system. And to say that I was having a hard time would be an understatement. It took me many PCRs, cloning and transformations to get to the right construct that would eventually express the desired…