How To Optimize Bioluminescence Assays for High-sensitivity Detection
To generate reliable quantitative bioluminescent data, you need more than a strong luciferase signal. Optimize bioluminescence assays for high-sensitivity detection by controlling substrate handling, timing, plate-reader settings, and normalization so technical noise doesn’t mask real biology. From luciferase and substrate choice to reader optimisation and in vivo bioluminescent imaging workflows, these practical strategies help you produce reproducible, interpretable data you can trust.
