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Are you stuck in cloning hell?, Tired of doing ligations that don’t work? Want a faster, more efficient cloning procedure? You should try ligation-independent cloning. A growing number of researchers swear by ligation-independent cloning methods because they are simpler and more efficient than conventional cloning and as a recent convert to their ranks, I’d like…
Traditionally, if you’re hoping to clone a DNA/RNA fragment (or insert) into a vector, such as a BAC you would need: This process looked something like this: cut you vector using exonucleases to expose ends that are complementary to the ends of your insert and then stick your insert into the vector using a ligase….
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